SA AS 5013191 2012-JUN-27 Food mcrobology Method 191 Mcrobology of food and anmal feedng stuffs-Horzontal method for the enumeraton of ß-glucurondase-postve Eschercha col-Colony-count technque at 44°C usng membranes and 5-bromo-4-chloro-3-ndolyl ß-D-glucuronde
This part of ISO 16649 specifies a horizontal method for the enumeration of -glucuronidase-positive Escherichia coli in products intended for human consumption or for the feeding of animals. It uses a colony-count technique after resuscitation using membranes and incubation at 44 °C on a solid medium containing a chromogenic ingredient for detection of the enzyme ß-glucuronidase.
WARNING — Strains of Escherichia coli which do not grow at 44 °C and, in particular, those that are ß-glucuronidase negative, such as Escherichia coli O157, will not be detected.
