DO-160G provides standard procedures and environmental test criteria for testing airborne equipment for the entire spectrum of aircraft from light, general aviation aircraft and helicopters through the "jumbo jets" and SST categories of aircraft. The document includes 26 sections and three appendices. Examples of tests covered include vibration, power input, radio frequency susceptibility, lightning and electrostatic discharge. Coordinated with EUROCAE, RTCA/DO-160G and EUROCAE/ED-14G are identically worded.

DO-160G is recognized by the International Organization for Standardization (ISO) as de facto international standard ISO-7137.