This European Standard specifies a method for the determination of monoacylglycerols, diacylglycerols and triacylglycerols and also free glycerol by high-performance size-exclusion chromatography. It is applicable to products (e.g. emulsifiers) comprising monoacylglycerols and diacylglycerols as main constituents in concentrations 10 %, and to triacylglycerols in a proportion of 20 %. The method is not applicable to dairy fats or fats and oils having a wide range of fatty acid chain lengths, since diacylglycerols of short fatty acids have a lower molecular mass than monoacylglycerols of long-chain fatty acids. The method has restricted applicability to acylglycerol mixtures based on caprylic and capric acids. Here, only the monoacylglycerol content and the free glycerol content can be determined.
NOTE: References [1] to [4] give background information.