This European Standard specifies a method for the determination of the mass fraction of d-biotin by high performance liquid chromatography (HPLC). The method has been validated in an inter-laboratory test on fortified and non-fortifed samples such as cereal breakfast powder, infant milk powder, lyophilized green peas with ham, lyophilized chicken soup and on nutritive orange juice, at levels from 16 μg/100 g to 200 μg/100 g. For further information on the validation data, see Annex B. NOTE 1 d-biocytin can also be estimated by this method. But none of the samples used for the validation step contained d-biocytin. Nonetheless the recovery rate is more than 90 % for d-biotin and d-biocytin, see [2] and [3]. NOTE 2 The method underestimates the real biotin content when used for samples containing egg.

Customers who purchased DS DS/EN 15607
also purchased

• DS DS/EN 1282-2 + A1 : Tracheostomy tubes - Part 2: Paediatric tubes
• DS DS/EN 15652 : Foodstuffs - Determination of niacin by HPLC
• SN NS-EN 14152:2003 : Foodstuffs - Determination of vitamin B2 by HPLC - (Corrigendum AC:2005 incorporated)

See More Documents